10         20         30         40         50
  M-KSPEEL KGIFEKYAAK EGDPNQLSKE ELKLLLQTEF PSLLKGPSTL 
        60         70 
DELFEELDKN GDGEVSFEEF QVLVKKISQ

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.

Supplied as
1.0 mg/mL solution in ammonium acetate 50 mM pH 6.0. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 1280 M-1cm-1 calculated).
(unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)

Characteristics
Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 2 mM.

Storage
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
NMR USE: after the experiments return the sample at 4°C.